Reporter | Enzyme Labeling | Detection Method | Mechanism Substrate | Forensic Application |
---|---|---|---|---|
AP | AP-conjugated probe | Chemiluminescent | Lumigen® PPD | RFLP; blot assay for DNA quantitation |
Horseradish Peroxidase (HRP) | Biotinylated probe, recognized by streptavidin conjugated with HRP | Colorimetric/ chemiluminescent | TMB/ luminol | Blot assay for DNA quantitation |
Horseradish Peroxidase (HRP) | Biotinylated probe, recognized by streptavidin conjugated with HRP | Colorimetric | TMB | Reverse blot assay for DQA1 typing and for mtDNA typing |
Source: Forensic Biology by Richard Li.