MethodForensic ApplicationPurityDNA StrandDNA SizeThroughput NoteAdditional Note
Solvent-basedRFLP- and PCR-based assayHighDouble strandedLargeTime-consuming; difficult to adapt for automationUses toxic solvent; multiple transfer steps between tubes
BoilingPCR-based assayLowSingle strandedSmall~30 min per sampleNo transfer required
Silica-basedRFLP- and PCR-based assayHighDouble strandedLarge~1 h per sample; amenable to automationMinimum transfer required

Source: Forensic Biology by Richard Li. Pg 113.

Leave a Reply