Method | Forensic Application | Purity | DNA Strand | DNA Size | Throughput Note | Additional Note |
---|---|---|---|---|---|---|
Solvent-based | RFLP- and PCR-based assay | High | Double stranded | Large | Time-consuming; difficult to adapt for automation | Uses toxic solvent; multiple transfer steps between tubes |
Boiling | PCR-based assay | Low | Single stranded | Small | ~30 min per sample | No transfer required |
Silica-based | RFLP- and PCR-based assay | High | Double stranded | Large | ~1 h per sample; amenable to automation | Minimum transfer required |
Source: Forensic Biology by Richard Li. Pg 113.