Assay TypeDescriptionApplications
Double antibody sandwich (DAS) ELISAAlso called direct ELISA. An antibody specific to the antigen is immobilized on a surface. The sample containing the antigen is added, followed by a labeled secondary antibody that also recognizes the antigen. This forms a “sandwich” of capture antibody-antigen-detection antibodies. [Image] Detection of specific antigens in a sample
Triple antibody sandwich (TAS) ELISAIt is also called indirect ELISA and is often used to identify antibodies in patient blood that may be there as the result of infection. [Image] Detection of low-abundance antigens, mainly for a diagnostic test for diseases such as HBV
Competitive ELISAThe antigen or antibody of interest in the sample competes with a labeled version for binding to a limited amount of immobilized antibody or antigen. The detection is based on the competition between the natural unlabelled antigen to be tested for a labeled form of the antigen which is the detection reagent. [Image] Detection of specific antigens or antibodies. Mainly used when the antigen is small and has only one epitope
Dissociation enhanced Lanthanide Fluorescence immunoassay (DELFIA)A time-resolved fluorescence immunoassay that uses lanthanide chelate antibody labels to generate a fluorescent signal when stimulated with the light of a specific wavelength. The light signal generated has a long decay which enhances the negative to the positive ration of the assay.Detection of specific antigens or antibodies, particularly in complex or autofluorescent samples


  • Principles and Techniques of Biochemistry and Molecular Biology by John Walker, Keith Wilson.

Leave a Reply