TechnologyAmplification methodSequencing methodInstrumentRead Length (bp)Runtime
454Emulsion PCRSynthesis
(pyrosequencing)
(i) 454-JS Junior
(ii) 454-FLX+
(i) 400
(ii) 700
(i) 10hrs
(ii) 23 hrs
IlluminaBridge PCRSynthesis(i) Illumina-MiSeq
(ii) Illumina-GAII
(iii) Illumina-HiScanSQ
(iv) Illumina-HISeq1000
(v) Illumina-HiSeq2000
(i) 150+150
(ii) 150+150
(iii) 100+100
(iv)100+100
(v) 100+100
(i) 27 hrs
(ii) 14 days
(iii) 11 days
(iv) 11 days
(v) 11 days
SOLiDEmulsion PCRLigation(i) SOLiD-5500
(ii) SOLiD-5500xl
(i) 75+35
(ii) 75+35
(i) 7 days
(ii) 7 days
Ion TorrentEmulsion PCRSynthesis
(H+ detection)
(i) Ion PGM-314 chip
(ii) Ion PGM- 316 chip
(iii) Ion PGM-318 chip
(i) 200
(ii) 200
(iii) 200
(i) 2hrs
(ii) 2hrs
(iii) 2hrs

Reference:

  • Encyclopedia of Forensic Sciences by Jay A. Siegel, Pekka J. Saukko
  • Websites:
    • Roche.com
    • Illumina.com
    • Lifetechnologies.com
    • Iontorrent.com

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